Modified coronally advanced flap technique in the treatment of fibrous epulis: A case report with 1-year follow-up.

BACKGROUND: Currently, the treatment of epulis is primarily surgical excision, which would greatly affect the aesthetics of patients if happened in the anterior region. It's challenging for clinicians to balance the aesthetic after surgery and less surgical trauma. To overcome this disadvantage, the authors propose the modified coronally advanced flap technique which applies the principles of minimally invasive surgery to provide satisfactory therapeutic results in fibrous epulis. METHODS: We report a case of an 18-year-old female with the chief complaint of a gingival swelling in the right upper anterior region. After the initial periodontal therapy, the modified surgical approach was applied to this patient. Unlike conventional coronally advanced flap technique, an additional incision was made, and the free portion was rotated into the adjacent space to completely cover the trauma, which avoided the use of the second operative zone. RESULTS: The gingiva recovered with normal color, contour, and consistency after surgery, the papilla filled up the proximal space well and was in good harmony with the adjacent papillae. The surgical results remained stable during the follow-up period. CONCLUSIONS: The use of modified coronally advanced flap technique allows the clinician to successfully resume the natural appearance of gingiva in the treatment of fibrous epulis, as well as simplify the surgical approach, shorten the operative time, and demonstrate no tendency of recurrence. KEY POINTS: Why is this case new information? This novel technique not only removes the epulis, but also takes into account the postoperative aesthetics of the surgery at the same time. This minimally invasive surgical technique reduces operative time and increases patient comfort. Keys to successful management of this case are as follows: (i) Adequate preoperative assessment of the location of the additional incision; (ii) tension-free coronal flap advancement. What are the primary limitations to success in this case? Clinical studies with long-term outcomes of this approach are needed. This procedure may be limited to larger gingival tumors.

Predicting sex differences in the effects of diuretics in renal epithelial transport during angiotensin II-induced hypertension.

Chronic angiotensin II (ANG II) infusion is an experimental model that induces hypertension in rodents. The natriuresis, diuresis, and blood pressure responses differ between males and females. This is perhaps not unexpected, given the rodent kidney, which plays a key role in blood pressure regulation, exhibits marked sex differences. Under normotensive conditions, compared with males, the female rat nephron exhibits lower Na+/H+ exchanger 3 (NHE3) activity along the proximal tubule but higher Na+ transporter activities along the distal segments. ANG II infusion-induced hypertension induces a pressure natriuretic response that reduces NHE3 activity and shifts Na+ transport capacity downstream. The goals of this study were to apply a computational model of epithelial transport along a rat nephron 1) to understand how a 14-day ANG II infusion impacts segmental electrolyte transport in male and female rat nephrons and 2) to identify and explain any sex differences in the effects of loop diuretics, thiazide diuretics, and K+-sparing diuretics. Model simulations suggest that the NHE3 downregulation in the proximal tubule is a major contributor to natriuresis and diuresis in hypertension, with the effects stronger in males. All three diuretics are predicted to induce stronger natriuretic and diuretic effects under hypertension compared with normotension, with relative increases in sodium excretion higher in hypertensive females than in males. The stronger natriuretic responses can be explained by the downstream shift of Na+ transport load in hypertension and by the larger distal transport load in females, both of which limit the ability of the distal segments to further elevate their Na+ transport.NEW & NOTEWORTHY Sex differences in the prevalence of hypertension are found in human and animal models. The kidney, which regulates blood pressure, exhibits sex differences in morphology, hemodynamics, and membrane transporter distributions. This computational modeling study provides insights into how the sexually dimorphic responses to a 14-day angiotensin II infusion differentially impact segmental electrolyte transport in rats. Simulations of diuretic administration explain how the natriuretic and diuretic effects differ between normotension and hypertension and between the sexes.

Single-Molecule Protein Analysis by Centrifugal Droplet Immuno-PCR with Magnetic Nanoparticles.

Detecting proteins in ultralow concentrations in complex media is important for many applications but often relies on complicated techniques. Herein, a single-molecule protein analyzer with the potential for high-throughput applications is reported. Gold-coated magnetic nanoparticles with DNA-labeled antibodies were used for target recognition and separation. The immunocomplex was loaded into microdroplets generated with centrifugation. Immuno-PCR amplification of the DNA enabled the quantification of proteins at the level of single molecules. As an example, ultrasensitive detection of α-synuclein, a biomarker for neurodegenerative diseases, is achieved. The limit of detection was determined to be ∼50 aM in buffer and ∼170 aM in serum. The method exhibited high specificity and could be used to analyze post-translational modifications such as protein phosphorylation. This study will inspire wider studies on single-molecule protein detection, especially in disease diagnostics, biomarker discovery, and drug development.

The impact of maxillary non-impacted third molars on the distal alveolar bone of adjacent teeth using CBCT: a retrospective study.

OBJECTIVE: The purpose of the study was to determine how the maxillary non-impacted third molars impact the distal region of alveolar bone of adjacent second molars. METHOD AND MATERIALS: The periodontal condition of maxillary second molars for which the neighboring third molars were missing (NM3- group) and those with intact non-impacted third molars (NM3+ group) was analyzed in a retrospective study. Using CBCT, the patients were categorized based on the presence or absence of periodontitis, and the alveolar bone resorption parameters in the distal area of the second molars were measured. RESULTS: A total of 135 patients with 200 maxillary second molars were enrolled in this retrospective study. Compared to the NM3- group, the second molars of the NM3+ group exhibited greater odds of increasing alveolar bone resorption in the distal region (health, OR = 3.60; periodontitis, OR = 7.68), regardless of the presence or absence of periodontitis. In healthy patients, factors such as female sex (OR = 1.48) and age above 25 years old (OR = 2.22) were linked to an elevated risk of alveolar bone resorption in the distal region of the second molars. In patients with periodontitis, male sex (OR = 3.63) and age above 45 years old (OR = 3.97) served as risk factors. CONCLUSIONS: Advanced age, sex, and the presence of non-impacted third molars are risk factors associated with alveolar bone resorption in individuals with adjacent second molars. In addition, the detrimental effects of non-impacted third molars in the population with periodontitis may be exacerbated. From a periodontal perspective, this serves as supportive evidence for the proactive removal of non-impacted third molars.

Inhibiting NLRP3 Inflammasome Activation by CY-09 Helps to Restore Cerebral Glucose Metabolism in 3×Tg-AD Mice.

The reduction of the cerebral glucose metabolism is closely related to the activation of the NOD-like receptor protein 3 (NLRP3) inflammasome in Alzheimer's disease (AD); however, its underlying mechanism remains unclear. In this paper, 18F-flurodeoxyglucose positron emission tomography was used to trace cerebral glucose metabolism in vivo, along with Western blotting and immunofluorescence assays to examine the expression and distribution of associated proteins. Glucose and insulin tolerance tests were carried out to detect insulin resistance, and the Morris water maze was used to test the spatial learning and memory ability of the mice. The results show increased NLRP3 inflammasome activation, elevated insulin resistance, and decreased glucose metabolism in 3×Tg-AD mice. Inhibiting NLRP3 inflammasome activation using CY-09, a specific inhibitor for NLRP3, may restore cerebral glucose metabolism by increasing the expression and distribution of glucose transporters and enzymes and attenuating insulin resistance in AD mice. Moreover, CY-09 helps to improve AD pathology and relieve cognitive impairment in these mice. Although CY-09 has no significant effect on ferroptosis, it can effectively reduce fatty acid synthesis and lipid peroxidation. These findings provide new evidence for NLRP3 inflammasome as a therapeutic target for AD, suggesting that CY-09 may be a potential drug for the treatment of this disease.

Enhanced methane production from anaerobic digestion of waste activated sludge by combining ultrasound with potassium permanganate pretreatment.

The influence of ultrasound (US) and potassium permanganate (KMnO4) co-pretreatment on anaerobic digestion of waste activated sludge (WAS) was investigated in this survey. Results showed that KMnO4 (0.3 g/g TSS) cooperated with US (1 W/mL, 15 min) pretreatment significantly increased the cumulative methane yield to 174.44 ± 3.65 mL/g VS compared to the control group (108.72 ± 2.56 mL/g VS), solo US (125.39 ± 2.56 mL/g VS), and solo KMnO4 pretreatment group (160.83 ± 1.61 mL/g VS). Mechanistic investigation revealed that US combined with KMnO4 pretreatment effectively disrupted the structure of extracellular polymeric substances and cell walls by generating reactive radicals, accelerating the release of organics and hydrolytic enzymes as well as improving the biodegradability of soluble organics. Modeling analysis illustrated that the biochemical methane potential and hydrolysis rate of WAS were enhanced under US + KMnO4 pretreatment. Microbial community distribution indicated that the co-pretreatment of US and KMnO4 elevated the total relative abundance of functional microorganisms associated with anaerobic digestion (22.01 %) compared to the control (10.69 %), US alone (12.24 %) and KMnO4 alone (16.20 %).

Enhanced anaerobic digestion of waste activated sludge with periodate-based pretreatment.

The potential of periodate (PI) in sludge anaerobic digestion is not tapped, although it has recently attracted great research interest in organic contaminants removal and pathogens inactivation in wastewater treatment. This is the first work to demonstrate significant improvement in methane generation from waste activated sludge (WAS) with PI pretreatment and to provide underlying mechanisms. Biochemical methane potential tests indicated that methane yield enhanced from 100.2 to 146.3 L per kg VS (VS, volatile solids) with PI dosages from 0 to 100 mg per g TS (TS, total solids). Electron spin resonance showed PI could be activated without extra activator addition, which might be attributed to the native transition metals (e.g., Fe2+) in WAS, thereby generating hydroxyl radical (•OH), superoxide radicals (•O2 -), and singlet oxygen (1O2). Further scavenging tests demonstrated all of them synergistically promoted WAS disintegration, and their contributions were in the order of •O2 - > •OH > 1O2, leading to the release of substantial biodegradable substances (i.e., proteins and polysaccharides) into the liquid phase for subsequent biotransformation. Moreover, fluorescence and ultraviolet spectroscopy analyses indicated the recalcitrant organics (especially lignocellulose and humus) could be degraded by reducing their aromaticity under oxidative stress of PI, thus readily for methanogenesis. Microbial community analysis revealed some microorganisms participating in hydrolysis, acidogenesis, and acetoclastic methanogenesis were enriched after PI pretreatment. The improved key enzyme activities and up-regulated metabolic pathways further provided direct evidence for enhanced methane production. This research was expected to broaden the application scope of PI and provide more diverse pretreatment choices for energy recovery through anaerobic digestion.

Enhancing methane production from waste activated sludge with heat-assisted potassium ferrate (PF) pretreatment: Reaction kinetics and mechanisms.

This work proposed a novel strategy via heat-assisted potassium ferrate (PF) pretreatment to enhance methane production from waste activated sludge (WAS) during anaerobic digestion. In this research, five dosages of PF (i.e., 0, 0.05, 0.1, 0.3 and 0.5 g/g VSS) at two temperatures (i.e., 25 °C and 55 °C) were explored. Biochemical methane potential experiments illustrated that heat-assisted PF pretreatment improved cumulative methane production with the maximum yield up to 163.93 mL CH4/g VSS, 149.0 %, 119.6 % and 121.0 % of that in the control, individual 0.5 g PF/g VSS and individual heat (i.e., 55 °C) pretreatment digesters, respectively. The maximum methane potential (B0) was promoted by 63.2 % with heat-assisted PF pretreatment compared to the control, while the hydrolysis rate (k) changed slightly. Mechanism analysis revealed that heat-assisted PF pretreatment accelerated WAS solubilization and enhanced the biodegradability of released substances, providing more available matrix for bacteria during the following anaerobic digestion processes. Microbial community analysis exhibited that several microbes such as Proteiniclasticum sp., Sedimentibacter sp. and Methanosaeta sp. associated with hydrolysis, acidification and methanogenesis respectively were improved after heat-assisted PF pretreatment. In addition, the relative bioactivities of protease, butyrate kinase and acetate kinase were also increased. Furthermore, variation of dominant genes associated with methane production indicated that acetate-dependent methanogenesis was the main pathway while CO2-dependent methanogenesis pathway was inhibited by heat-assisted PF pretreatment.

Ultrasound-sodium percarbonate effectively promotes short-chain carboxylic acids production from sewage sludge through anaerobic fermentation.

Short-chain carboxylic acids (SCCAs) production from sewage sludge via anaerobic fermentation is usually restricted by low substrates availability and rapid products consumption. Therefore, the ultrasound (US)-sodium percarbonate (SPC) technique was proposed to effectively break the bottlenecks. Results showed the total SCCAs yield, acetate yield and particulate organics reduction respectively attained 392.8 mg COD/g VSS, 204.6 mg COD/g VSS and 47.4 % under the optimal condition. Mechanistic explorations disclosed that US + SPC largely reduced biodegradation resistances of particulate organics and improved sludge biodegradability. The destruction of spatial structure was the inherent mechanisms for initial solubilization and further degradation of solid-phase sludge. Besides, US + SCP up-regulated hydrolytic and SCCAs-forming enzymes, but downregulated the key enzyme for methanation. Meanwhile, US + SPC altered the microbial structure and stimulated functional microorganism enrichment, well correlated with substrate biotransformation and products output. Overall, this strategy could effectively enhance SCCAs production from WAS and reduce the environmental risk for subsequent sludge disposal.

Potassium permanganate pretreatment effectively improves methane production from anaerobic digestion of waste activated sludge: Reaction kinetics and mechanisms.

As a powerful oxidizing agent, potassium permanganate (KMnO4) has attracted widespread interest in sludge treatment and contaminant removal. However, its effect on the anaerobic digestion of waste activated sludge (WAS) is ambiguous. This investigation was designed to provide perspectives into this problem. In comparison with the control, 0.3 g KMnO4/g TSS pretreatment enhanced the methane production by 78.82 %. Model analysis demonstrated that the KMnO4 pretreatment enhanced the biochemical methane potential (B0) of WAS. Mechanistic studies elucidated that the KMnO4 pretreatment process generated reactive radicals such as ·OH, ·O2- and 1O2, which contributed to sludge disintegration and biodegradation process of dissolved substances, thus resulting in more substances available for subsequent methane generation. Enzyme activity analysis indicated that KMnO4 pretreatment facilitated the activities of key enzymes associated with anaerobic digestion to various degrees. Microbial analysis illustrated that the relative abundance of functional microorganisms was significantly elevated after KMnO4 pretreatment, which was conducive to methane production.

Performance and mechanism of sodium percarbonate (SPC) enhancing short-chain fatty acids production from anaerobic waste activated sludge fermentation.

A novel pretreatment technique (i.e., using Sodium percarbonate, SPC) to improve the short-chain fatty acids (SCFA) production waste activated sludge (WAS) was proposed in this study. Results indicated that the maximum SCFA production of 1605.7 mg COD/L and acetic acid of 52.9% were attained at 0.2 g SPC/g TSS, being 8.4 and 2.8 times of the control (191.3 mg COD/L and 19%), respectively. Meanwhile, the optimal time for SCFA accumulation was decreased from 6d (control) to 4d (0.2 g/g TSS). Mechanism explorations unraveled that SPC largely accelerated WAS solubilization and enhanced the bioavailability of organics released from WAS. It improved enzymatic activities related to hydrolysis and acidogenesis, while suppressed the Coenzyme F420 responsible for SCFA consumption. Illumina MiSeq sequencing analysis showed that SPC substantially enhanced the relative abundances of hydrolytic and/or acid-forming microbes. Furthermore, CO3- and O2- were the key factors to production enhancement in SPC-involved sludge fermentation.

Unveiling the mechanisms of how vivianite affects anaerobic digestion of waste activated sludge.

Recently, phosphorus recovery as vivianite from sludge digestion system has attracted increasing attention because of its high recovery efficiency and economic value. However, the potential impact of vivianite on anaerobic digestion of waste activated sludge remains largely unknown. This study therefore aims to provide such support. Experimental results revealed that the maximal methane yield decreased from 103.55 to 76.55 mL/g volatile solids, with the vivianite level increasing from 0 to 500 mg P/L. Mechanism explorations showed that vivianite caused more substrates remaining in tightly-bound extracellular polymeric substances, and thus suppressed sludge solubilization. In addition, it was observed that hydrolysis, acidiogenesis, acetogenesis and methanogenesis bio-processes were all inhibited by vivianite. Microbial analysis revealed that vivianite significantly decreased the relative abundances of hydrolytic microbes, acidogens and methanogens. Further investigation showed that vivianite benefited sludge agglomeration and can enhance the mass transfer resistance of anaerobic digestion, further supporting the inhibitions on anaerobic digestion.

Pipette-Tip-Enabled Digital Nucleic Acid Analyzer for COVID-19 Testing with Isothermal Amplification.

Herein, a pipette-tip-enabled digital nucleic acid analyzer for high-performance COVID-19 testing is demonstrated. This is achieved by digital loop-mediated isothermal amplification (digital LAMP or dLAMP) using common laboratory equipment and materials. It is shown that simply fixing a glass capillary inside conventional pipette tips enables the generation of monodisperse, water-in-oil microdroplets with benchtop centrifugation. It is shown that using LAMP, the ORF1a/b gene, a standard test region for COVID-19 screening, can be amplified without a thermal cycler. The amplification allows counting of fluorescent microdroplets so that Poisson analysis can be performed to allow quantification with a limit of detection that is 1 order of magnitude better than those of nondigital techniques and comparable to those of commercial dLAMP platforms. It is envisioned that this work will inspire studies on ultrasensitive digital nucleic acid analyzers demanding both sensitivity and accessibility, which is pivotal to their large-scale applications.

Development, Identification, and Application of a Germplasm Specific SCAR Marker for Dendrobium officinale Kimura et Migo.

The stems of Dendrobium officinale have been used as a rare and valuable Chinese tonic medicine, known as "Tiepi Fengdou", since the Qing dynasty. Because of the increased market demand and continued exploitation of this plant, the reserves of wild D. officinale resources have been depleted, and D. officinale products on the market are being increasingly adulterated. Such changes have strongly affected the sustainable utilization of this valuable medicinal plant resource and the development of related industries. In this study, a species-specific DNA marker was developed for the rapid and accurate authentication of D. officinale. In total, 36 start codon-targeted (SCoT) polymorphism primers were screened in 36 definite Dendrobium species, and a distinct species-specific DNA amplicon (SCoT13-215) for D. officinale was obtained. After the sequence was cloned and sequenced, a sequence-characterized amplified region marker was developed (named SHF/SHR) and validated through PCR amplification of all 38 Dendrobium samples. The marker's specificity for D. officinale was confirmed through the consistent amplification of a clear 197-bp band. This SCAR marker can be used to rapidly, effectively, and reliably identify D. officinale among various Dendrobium species and may play an important role in ensuring the quality of medicinal preparations and protecting the germplasm of this important medicinal species.

Complete chloroplast genomes of four Physalis species (Solanaceae): lights into genome structure, comparative analysis, and phylogenetic relationships.

BACKGROUND: Physalis L. is a genus of herbaceous plants of the family Solanaceae, which has important medicinal, edible, and ornamental values. The morphological characteristics of Physalis species are similar, and it is difficult to rapidly and accurately distinguish them based only on morphological characteristics. At present, the species classification and phylogeny of Physalis are still controversial. In this study, the complete chloroplast (cp) genomes of four Physalis species (Physalis angulata, P. alkekengi var. franchetii, P. minima and P. pubescens) were sequenced, and the first comprehensive cp genome analysis of Physalis was performed, which included the previously published cp genome sequence of Physalis peruviana. RESULTS: The Physalis cp genomes exhibited typical quadripartite and circular structures, and were relatively conserved in their structure and gene synteny. However, the Physalis cp genomes showed obvious variations at four regional boundaries, especially those of the inverted repeat and the large single-copy regions. The cp genomes' lengths ranged from 156,578 bp to 157,007 bp. A total of 114 different genes, 80 protein-coding genes, 30 tRNA genes, and 4 rRNA genes, were observed in four new sequenced Physalis cp genomes. Differences in repeat sequences and simple sequence repeats were detected among the Physalis cp genomes. Phylogenetic relationships among 36 species of 11 genera of Solanaceae based on their cp genomes placed Physalis in the middle and upper part of the phylogenetic tree, with a monophyletic evolution having a 100% bootstrap value. CONCLUSION: Our results enrich the data on the cp genomes of the genus Physalis. The availability of these cp genomes will provide abundant information for further species identification, increase the taxonomic and phylogenetic resolution of Physalis, and assist in the investigation and utilization of Physalis plants.

Impact of an Information Leaflet on Knowledge of Hepatocellular Carcinoma and Hepatitis B among Chinese Youth.

BACKGROUND: To assess the effect of an information leaflet on the level of Chinese youth's knowledge about hepatitis B and hepatocellular carcinoma (HCC), the most common type of primary liver cancer (PLC). MATERIALS AND METHODS: A total of 500 students, from two universities in the Chaoshan area of China, were randomly divided into an intervention group of 280 participants and a control group of 220. Baseline knowledge of HCC and hepatitis B was evaluated by questionnaire interview. Subsequently, only the intervention group was given an information leaflet of HCC and hepatitis B. Three months later, the two groups were contacted for a second interview. Changes in knowledge from baseline of HCC and hepatitis B were compared between the two groups. RESULTS: There was no statistically significant difference in mean PRE-questionnaire scores between the intervention and control groups. However, the mean POST-questionnaire score was significantly higher in the intervention group after the intervention. The leaflet had the greatest effect on the participants' questionnaire score, and raised their level of knowledge about HCC and hepatitis B. CONCLUSIONS: The information leaflet intervention is significantly effective in improving the knowledge of HCC and hepatitis B among the youth.